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Genetic analyzer Nanophore 05 as a measuring instrument for DNA sequencing
https://doi.org/10.32446/0368-1025it.2021-1-60-65
Abstract
The paper presents the results of studies on the development of metrological support for DNA sequencing. The sequencing of a standard sample of the human mitochondrial DNA was carried out according to the Sanger method on domestic capillary electrophoresis genetic analyzers Nanophore 05. The results of the work were used to validate standard reference material for human mitochondrial DNA sequence and to confi rm the DNA sequencer as a measurement instrument.
About the Authors
A. A. Volkov
Russian Federal Research Institute of Fisheries and Oceanography
Russian Federation
Russian Federation
Aleksandr A. Volkov
Moscow
I. A. Volkov
Limited Liability Company Syntol
Russian Federation
Russian Federation
Il’ya A. Volkov
Moscow
A. G. Plugov
Limited Liability Company Syntol
Russian Federation
Russian Federation
Aleksandr G. Plugov
Moscow
E. V. Kulyabina
All-Russian Research Institute of Metrological Service
Russian Federation
Russian Federation
Elena V. Kulyabina
Moscow
O. N. Melkova
All-Russian Research Institute of Metrological Service
Russian Federation
Russian Federation
Olga N. Melkova
Moscow
G. S. Lavrov
Institute for Analytical Instrumentation, Russian Academy of Sciences
Russian Federation
Russian Federation
Georgii S. Lavrov
St. Petersburg
D. V. Bocharova
Institute for Analytical Instrumentation, Russian Academy of Sciences
Russian Federation
Russian Federation
Dariya V. Bocharova
St. Petersburg
Y. I. Alekseev
Limited Liability Company Syntol;
Institute for Analytical Instrumentation, Russian Academy of Sciences
Russian Federation
Russian Federation
Yakov I. Alekseev
Moscow;
St. Petersburg
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Review
For citations:
Volkov A.A., Volkov I.A., Plugov A.G., Kulyabina E.V., Melkova O.N., Lavrov G.S., Bocharova D.V., Alekseev Y.I. Genetic analyzer Nanophore 05 as a measuring instrument for DNA sequencing. Izmeritel`naya Tekhnika. 2021;(1):60-65. (In Russ.) https://doi.org/10.32446/0368-1025it.2021-1-60-65
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