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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">izmertech</journal-id><journal-title-group><journal-title xml:lang="ru">Измерительная техника</journal-title><trans-title-group xml:lang="en"><trans-title>Izmeritel`naya Tekhnika</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">0368-1025</issn><issn pub-type="epub">2949-5237</issn><publisher><publisher-name>ФГУП "ВНИИФТРИ"</publisher-name></publisher></journal-meta><article-meta><article-id custom-type="elpub" pub-id-type="custom">izmertech-176</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>МЕДИЦИНСКИЕ И БИОЛОГИЧЕСКИЕ ИЗМЕРЕНИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>MEDICAL AND BIOLOGICAL MEASUREMENTS</subject></subj-group></article-categories><title-group><article-title>Определение метрологических характеристик сканирующего ближнепольного оптического микроскопа при исследовании биологических объектов</article-title><trans-title-group xml:lang="en"><trans-title></trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Булыгин</surname><given-names>Ф. В.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Драчева</surname><given-names>О. Е.</given-names></name></name-alternatives><email xlink:type="simple">o.dracheva@vniiofi.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Кутузов</surname><given-names>Н. П.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Лясковский</surname><given-names>В. Л.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Максимов</surname><given-names>Г. В.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Николаев</surname><given-names>Ю. А.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-2"/></contrib></contrib-group><aff xml:lang="ru" id="aff-1"><institution>Всероссийский научно-исследовательский институт оптико-физических измерений</institution><country>Russian Federation</country></aff><aff xml:lang="ru" id="aff-2"><institution>Московский государственный университет имени М. В. Ломоносова</institution><country>Russian Federation</country></aff><pub-date pub-type="collection"><year>2013</year></pub-date><pub-date pub-type="epub"><day>07</day><month>02</month><year>2023</year></pub-date><volume>0</volume><issue>10</issue><fpage>55</fpage><lpage>59</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; ФГУП "ВНИИФТРИ", 2023</copyright-statement><copyright-year>2023</copyright-year><copyright-holder xml:lang="ru">ФГУП "ВНИИФТРИ"</copyright-holder><copyright-holder xml:lang="en">ФГУП "ВНИИФТРИ"</copyright-holder><license xlink:href="https://www.izmt.ru/jour/about/submissions#copyrightNotice" xlink:type="simple"><license-p>https://www.izmt.ru/jour/about/submissions#copyrightNotice</license-p></license></permissions><self-uri xlink:href="https://www.izmt.ru/jour/article/view/176">https://www.izmt.ru/jour/article/view/176</self-uri><abstract><p>Предложены методы определения основных метрологических характеристик сканирующего ближнепольного оптического микроскопа – масштабного коэффициента и пространственного разрешения, характеризующегося как диаметр оптического зонда. Исследована возможность использования предложенного метода для калибровки сканирующего ближнепольного оптического микроскопа непосредственно в процессе измерений параметров биологических объектов.</p></abstract><trans-abstract xml:lang="en"><p>The methods of the scanning near-field optical microscope main metrological characteristics determination are proposed. These characteristics include a scale factor and a spatial resolution characterized as an optical probe diameter. The possibility of the proposed method usage for scanning near-field optical microscope calibration directly during the biological objects parameters measurement has been studied.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>сканирующий ближнепольный оптический микроскоп</kwd><kwd>масштабный коэффициент</kwd><kwd>диаметр оптического зонда</kwd><kwd>near-field scanning optical microscope</kwd><kwd>scale factor</kwd><kwd>optical probe diameter</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Koopman M. e. a. Near-field scanning optical microscopy in liquid for high resolution single molecule detection on dendritic cells // FEBS Lett. 2004. N 573. P. 6–10.</mixed-citation><mixed-citation xml:lang="en">Koopman M. e. a. Near-field scanning optical microscopy in liquid for high resolution single molecule detection on dendritic cells // FEBS Lett. 2004. N 573. 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